浩瀚1.5%OV-17/Shimalite W填充柱测定维生素2m*3mm

【含量测定】 照气相色谱法（附录Ⅴ E）测定。
色谱条件与系统适用性试验 以硅酮(OV-17) 为固定相，涂布浓度为2 ％；柱温为
265 ℃。理论板数按维生素Ｅ峰计算应不低于500 ，维生素Ｅ峰与内标物质峰的分离度
应大于2 。
校正因子测定 取正三十二烷适量，加正己烷溶解并稀释成每1ml 中含 1.0mg的溶
液，摇匀，作为内标溶液。另取维生素Ｅ对照品约20mg，精密称定，置棕色具塞锥形瓶
中，精密加入内标溶液10ml，密塞，振摇使溶解；取1 ～3μl注入气相色谱仪，计算校
正因子。
测定法 取本品约20mg，精密称定，置棕色具塞锥形瓶中，精密加入内标溶液10ml
，密塞，振摇使溶解；取1 ～3μl注入气相色谱仪，测定，计算，即得。

名称：不锈钢柱
规格：2m*3mm，60-80目
型号：1.5%OV-17/Shimalite  W
编号：20230914001
*高温度：300°C
 
【 Content determination 】 Determine according to the gas chromatography method (Appendix V E).

Chromatographic conditions and system suitability tests were conducted using silicone (OV-17) as the stationary phase with a coating concentration of 2%; Column temperature is

265 ℃. The number of theoretical plates should not be less than 500 calculated based on the vitamin E peak, and the separation degree between the vitamin E peak and the internal standard substance peak should not be less than 500

Should be greater than 2.

Correction factor determination: Take an appropriate amount of n-hexane, dissolve it with n-hexane, and dilute it to a solution containing 1.0mg per 1ml

Shake well and use as the internal standard solution. Take approximately 20mg of vitamin E reference material, accurately weigh it, and place it in a brown conical flask with a stopper

Add 10ml of internal standard solution accurately, seal tightly, shake to dissolve; Take 1-3 μ Inject gas chromatograph, calculate and calibrate

Positive factor.

Determination method: Take approximately 20mg of this product, accurately weigh it, place it in a brown conical flask with a stopper, and accurately add 10ml of internal standard solution

Densify and shake to dissolve; Take 1-3 μ Inject into a gas chromatograph, measure, calculate, and obtain.



Name: Stainless steel column

Specification: 2m * 3mm, 60-80 mesh

Model: 1.5% OV-17/Shimalite W

No.: 20230914001

Maximum temperature: 300 ° C